Article Figures & Data
Figures
- Figure 1.
Time course of the changes in proliferative response to 17β-oestradiol following long-term maintenance of MCF-7 human breast cancer cells with and without oestradiol or insulin. Cells were maintained for 26 weeks in phenol-red-free Dulbecco's modified Eagle's medium (DMEM) / 5% dextran charcoal-stripped foetal calf serum (DCFCS) with 10−8 M 17β-oestradiol and 10 μg/ml insulin (stock 1), with 10−8 M 17β-oestradiol only (stock 2), with 10 μg/ml insulin only (stock 3), or with no addition (stock 4). At increasing time points, proliferation of the cells was assayed as the number of cell doublings over 14 days in the same medium with no addition (no(-)E) or with 10−8 M 17β-oestradiol (+E). Error bars are the standard error of all nine values of triplicate estimates of cell numbers after 0 and 14 days, where no bar is seen, the error was too small for visual display.
- Figure 2.
Time course of the changes in proliferative response to insulin following long-term maintenance of MCF-7 human breast cancer cells with and without oestradiol or insulin. Cells were maintained for 26 weeks in phenol-red-free Dulbecco's modified Eagle's medium (DMEM) / 5% dextran charcoal-stripped foetal calf serum (DCFCS) with 10−8 M 17β-oestradiol and 10 μg/ml insulin (stock 1), with 10−8 M 17β-oestradiol only (stock 2), with 10 μg/ml insulin only (stock 3), or with no addition (stock 4). At increasing time points, proliferation of the cells was assayed as number of cell doublings over 14 days in the same medium with no addition (no(-)Ins) or with 10 μg/ml insulin (+Ins). Error bars are the standard error of all nine values of triplicate estimates of cell numbers after 0 and 14 days, where no bar is seen, the error was too small for visual display.
- Figure 3.
Regulation of a transiently-transfected oestrogen-inducible luciferase (ERE-LUC) reporter gene (A) and levels of oestrogen receptor alpha (ERα) protein (B) following long-term maintenance of MCF-7 human breast cancer cells with and without oestradiol or insulin. Cells were maintained for 28 weeks in phenol-red-free Dulbecco's modified Eagle's medium (DMEM)/5% dextran charcoal-stripped foetal calf serum (DCFCS) with 10−8 M 17β-oestradiol and 10 μg/ml insulin (stock 1), with 10−8 M 17β-oestradiol only (stock 2), with 10 μg/ml insulin only (stock 3), or with no addition (stock 4). A: Induction of ERE-LUC was assayed in the same medium with no addition (-E) or with 10−8 M 17β-oestradiol (+E) and results presented as the ratio of firefly luciferase:renilla luciferase. Error bars are the standard error of triplicate wells. B: ERα was measured by western immunoblotting.
- Figure 4.
Development of hypersensitivity to oestradiol for proliferation in serum-free conditions following long-term maintenance of MCF-7 human breast cancer cells with and without oestradiol or insulin. Cells were maintained for 30 weeks in phenol-red-free Dulbecco's modified Eagle's medium (DMEM) / 5% dextran charcoal-stripped foetal calf serum (DCFCS) with 10−8 M 17β-oestradiol and 10 μg/ml insulin (stock 1), with 10−8 M 17β-oestradiol only (stock 2), with 10 μg/ml insulin only (stock 3), or with no addition (stock 4). Proliferation was assayed under serum-free conditions and results are presented as number of cell doublings in 6 days at the indicated concentrations of 17β-oestradiol. Error bars are the standard error of all nine values of triplicate estimates of cell numbers after 0 and 6 days, where no bar is seen, the error was too small for visual display.
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