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Research ArticleExperimental Studies

Stable Knockdown of hCGβ mRNA Expression in Bladder Cancer Cells Results in Significant Growth Inhibition

BEATA BURCZYNSKA, MATTHEW J. BOOTH, RAY K. ILES, ANKITA SHAH, ASHREF SHILED and STEPHEN A. BUTLER
Anticancer Research September 2013, 33 (9) 3611-3614;
BEATA BURCZYNSKA
1Center for Investigative and Diagnostic Oncology, Middlesex University, London, U.K.
4Department of Cell Biology, Poznan University of Medical Sciences, Poznan, Poland
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MATTHEW J. BOOTH
2Institute for Health Research and Policy, School of Human Sciences, London Metropolitan University, Holloway Road, London, U.K.
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RAY K. ILES
1Center for Investigative and Diagnostic Oncology, Middlesex University, London, U.K.
3ELK Foundation for Health Research, Crieff, Scotland, U.K.
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  • For correspondence: ray@iles.net
ANKITA SHAH
1Center for Investigative and Diagnostic Oncology, Middlesex University, London, U.K.
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ASHREF SHILED
1Center for Investigative and Diagnostic Oncology, Middlesex University, London, U.K.
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STEPHEN A. BUTLER
1Center for Investigative and Diagnostic Oncology, Middlesex University, London, U.K.
2Institute for Health Research and Policy, School of Human Sciences, London Metropolitan University, Holloway Road, London, U.K.
3ELK Foundation for Health Research, Crieff, Scotland, U.K.
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Abstract

Background: Expression of human chorionic gonadotropin beta subunit (hCGβ) by epithelial carcinomas is associated with a poor prognosis and has a proposed autocrine growth effect on cancer cells by inhibition of apoptosis. Material and Methods: We transduced the hCGβ-expressing bladder cancer cell line SCaBER with short hairpin (sh) RNA lentiviral gene-specific (CGB) constructs and determined its impact on the synthesis of hCGβ and the resultant effect on cancer cell growth. Results: Stable CGB gene-silenced clones exhibited a 60%-80% reduction in the level of hCGβ expressed and a reduced growth rate of more than 40% compared to wild-type SCaBER cells. Conclusions: shRNA Lentiviral particles achieve stable knockdown of hCGβ translation in the bladder cancer cell line SCaBER. This transforms the phenotype by reducing hCGβ expression and cell growth rate. This is consistent with the proposed autocrine/paracrine function of ectopic hCGβ expression during oncogenesis.

  • Lentiviral siRNA
  • hCGβ
  • bladder carcinoma
  • silencing
  • stable gene knockdown SCaBER cells
  • Received June 19, 2013.
  • Revision received July 16, 2013.
  • Accepted July 17, 2013.
  • Copyright© 2013 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Anticancer Research: 33 (9)
Anticancer Research
Vol. 33, Issue 9
September 2013
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Stable Knockdown of hCGβ mRNA Expression in Bladder Cancer Cells Results in Significant Growth Inhibition
BEATA BURCZYNSKA, MATTHEW J. BOOTH, RAY K. ILES, ANKITA SHAH, ASHREF SHILED, STEPHEN A. BUTLER
Anticancer Research Sep 2013, 33 (9) 3611-3614;

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Stable Knockdown of hCGβ mRNA Expression in Bladder Cancer Cells Results in Significant Growth Inhibition
BEATA BURCZYNSKA, MATTHEW J. BOOTH, RAY K. ILES, ANKITA SHAH, ASHREF SHILED, STEPHEN A. BUTLER
Anticancer Research Sep 2013, 33 (9) 3611-3614;
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Keywords

  • Lentiviral siRNA
  • hCGβ
  • bladder carcinoma
  • silencing
  • stable gene knockdown SCaBER cells
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