Article Figures & Data
Figures
- Figure 1.
Immunocytochemical detection of some tumor markers. A-H: Histochemistry of malignant tissue (A-D) and normal tissue (E-H). Sections were stained with hematoxylin and eosin (A and E), or immunohistochemically stained for cytokeratin-19 (CK19; B and F), mucin-1 (MUC1; C and G), and breast cancer antigen 225 (BCA225; D and H). Immunostained tissues were double stained with DAPI (2 μg/ml). Mammary ducts were rich in nuclei (F-H) and negative for the proteins examined. I-L: Primary cultured MCKH cells were photographed under phase-contrast microscopy (I) or immunohistochemically stained for CK19 (J and K) and tubulin (L). CK19 staining was positive in early-passage cells (J: population doubling level=1.68) and negative in aged cells (K: population doubling level=9.43). Scale bar=100 μm.
- Figure 2.
Survival rate and heat shock protein gene expression after heat shock treatment. A: Relative viability 24 h after heat-shock. After heat shock at 43°C for 1 h, the difference between CRL-8798 and MCKH cells was significant (*p=0.036). For the MCKH cells, the difference between heat shock at 43°C for 10 min and 43°C for 1 h was also significant (**p=0.039). Significant differences were calculated with Student's t-test. B-D: Relative expression of heat-shock protein genes, 1 h after heat shock in HDF (B), CRL-8798 (C), and MCKH (D) cells.
