Figure 5.
Quercetin affected on the levels of proteins associated with migration and invasion in SAS cells. Cells were treated with 0, 25 and 50 μM of quercetin for 24 and 48 h and then harvested. The total proteins from each treatment were collected and the proteins levels (A: matrix metalloproteinase (MMP)-2, -7, -9 and -10 and vascular endothelial growth factor (VEGF); B: nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, inductible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and urokinase-type plasminogen activator (uPA); C: phosphatidylinositide 3-kinases (PI3K), nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IKBα), IKB-α/β, phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor kinase, alpha/beta (p-IKKα/β); D: focal adhesion kinase (FAK), son of sevenless homolog-1 (SOS1), Ras homolog gene family, member A (RhoA), growth factor receptor-bound protein-2 (GRB2), protein kinase C (PKC), rat sarcoma viral oncogene homolog (RAS), mitogen-activated protein kinase kinase kinase-3 (MEKK3) and MEKK7; E: extracellular-signal-regulated kinase 1/2 (ERK1/2), phosphorylated-extracellular-signal-regulated kinase 1/2 (p-ERK1/2), c-Jun N-terminal kinase 1/2 (JNK1/2), p38, p-p38, Jun proto-oncogene (c-JUN) and phosphorylated-Jun proto-oncogene (p-c-JUN), were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting, as described in Materials and Methods.