Article Figures & Data
Figures
- Figure 1.
Experimental schema for Gelfoam® angiogenesis. Gelfoam® (5×5 mm), treated with 300 ng β-fibroblast growth factor in 75 μl RPMI-1640 medium, was transplanted into the subcutis on both flanks of red fluorescent protein (RFP) transgenic nude mice. Skin flaps were made at day 7 after transplantation of Gelfoam® under anesthesia. Angiogenesis was observed in the Gelfoam® in the skin flap using confocal microscopy.
- Figure 2.
Vascularization of implanted Gelfoam® with RFP-expressing vessels. Seven days after Gelfoam® transplantation, skin flaps were made and observed with the FV1000 laser scanning confoal microscope. A: Seven days after Gelfoam® transplantation, RFP-expressing cells infiltrated the Gelfoam®, but no vessel structures were observed. Bar=200 μm. B: Fourteen days after transplantation, RFP-expressing vessel structures were observed on the surface of Gelfoam®. Bar=200 μm. C: Twenty-one days after transplantation, RFP-expressing vessels were observed invading the Gelfoam®. Bar=200 μm. D: Twenty-eight days after transplantation, RFP-expressing vessels were thick and long and invaded the Gelfoam®. “V” in the figure indicates blood vessels. Bar=200 μm.
- Figure 3.
Co-localization of RFP and CD31 in blood vessels in Gelfoam®. Frozen sections were made at day 28 after transplantation of Gelfoam®, treated with β-fibroblast growth factor, into the subcutis of RFP transgenic nude mice. A: RFP-expressing blood vessels were growing into the Gelfoam®. “V” indicates blood vessels. B: Immunohistochemical staining showed that CD31 and RFP fluorescence co-localized in the RFP-expressing blood vessels. Arrows indicate where blood vessels are located.
- Figure 4.
Behavior of αv integrin GFP-expressing 143B cells in vitro. A: 143B cells stably expressing αv integrin-GFP in vitro. Bar=200 μm. B: Punctate expression of αv integrin-GFP was observed at the bottom of the dish when 143B cells were seeded on a fibronectin-coated dish. Bar=50 μm.
- Figure 5.
In vivo visualization of the interaction between 143B cells expressing αv integrin GFP and RFP vessels in Gelfoam®. Skin flaps were made seven days after transplantation of 143B cancer cells (5×105), expressing αv integrin-GFP, into the Gelfoam®, previously transplanted in RFP transgenic nude mice. The skin flaps were closed with a 6-0 nylon suture. A: Seven days after cell injection, GFP-expressing cancer cells were observed in the Gelfoam®. Bar=100 μm. B: Fourteen days after injection, GFP-expressing cancer cells were found proliferating in the Gelfoam®. Bar=100 μm. C: Twenty-one days after injection, αv integrin-GFP-expressing cancer cells were found to be proliferating in the Gelfoam®. αv Integrin-GFP was strongly expressed around the surface of the cells. Bar=100 μm. D: Twenty-eight days after injection, cancer cells strongly expressed αv integrin-GFP around a small RFP-expressing vessel (V). Bar=100 μm.
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