Correlation of Aquaporin-1 Water Channel Protein Expression with Tumor Angiogenesis in Human Astrocytoma

NICOLAI EL HINDY; AGNES BANKFALVI; ARNE HERRING; MICHAEL ADAMZIK; NICOLE LAMBERTZ; YUAN ZHU; WINFRIED SIFFERT; ULRICH SURE; I. EROL SANDALCIOGLU

Article Figures & Data

Figures

Tables

Download figure
Open in new tab
Download powerpoint
Figure 1.
Immunhistochemical staining (brown) for Aquaporin1 (AQP1). Normal brain (A) revealed solely capillary staining of AQP1 (*). Astrocytoma grade II (B) exhibited mainly membranous staining of some tumour cells, presenting the typical astrocytic appearance (*). Anaplastic astrocytoma grade III (C) revealed denser AQP1 staining of tumour cells. Besides membranous staining, intracytoplasmatic staining was stronger (*). Grade IV astrocytoma (D) exhibited massive membranous and intracytoplasmatic AQP1 expression, with a lack of endothelial expression (*).
Download figure
Open in new tab
Download powerpoint
Figure 2.
Immunhistochemical staining of Aquaporin1 (AQP1) (brown, A, B, D, F), and laminin (blue, C, E) in grade IV astrocytoma: The difference between massive AQP1 up-regulation (A) in tumour (T) and lack of expression in normal brain (N) was obvious. The infiltration zone (B) revealed expression of AQP1 similar to that of low-grade astrocytoma (I) in contrast to dense tumour (T). Laminin revealed two different neovascularization patterns: Glomeruloid neovascularization was mainly found within the necrotic tumour core (C). Many branching, connected microvessels were found distant from the tumour core in the periphery, near infiltrating areas (E). There was a remarkable difference in AQP1 expression among the different patterns. Great overexpression was visible, especially perivascularly in the tumour periphery (F), in contrast to only weak AQP1 expression in the necrotic tumour core (D).