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Research Article

Anticancer Effects of Eleven Triterpenoids Derived from Antrodia camphorata

YI-PANG LEE, WAN-CHI TSAI, CHIH-JAN KO, YERRA KOTESWARA RAO, CHIUNG-RU YANG, DAR-REN CHEN, MING-HUI YANG, CHI-CHIANG YANG and YEW-MIN TZENG
Anticancer Research July 2012, 32 (7) 2727-2734;
YI-PANG LEE
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WAN-CHI TSAI
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CHIH-JAN KO
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YERRA KOTESWARA RAO
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CHIUNG-RU YANG
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DAR-REN CHEN
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MING-HUI YANG
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CHI-CHIANG YANG
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  • For correspondence: cyang@csmu.edu.tw ymtzeng@cyut.edu.tw
YEW-MIN TZENG
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  • For correspondence: cyang@csmu.edu.tw ymtzeng@cyut.edu.tw
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  1. Figure 1.
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    Figure 1.

    Chemical structures of triterpenoid compounds 1-11 isolated from the fruiting bodies of Antrodia camphorata.

  2. Figure 2.
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    Figure 2.

    Morphological change in PC-3 cells induced by treatment with triterpenoid compounds 1-11. PC-3 cells were treated with different compounds for 24 hours. Cell morphology was observed under optical microscopy at ×100 (A) and ×200 (B) magnification. Ctrl: DMSO vehicle control; Camp.: camptothecin, positive control. For compound names, see legend to Figure 1.

  3. Figure 3.
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    Figure 3.

    Effects of triterpenoid compounds 1-11 on caspase-3 activity in PC-3 cells. PC-3 cells were treated with 50 μM of each compound or camptothecin (Camp.), which served as the positive control. Flow cytometric analysis was carried out after 24-h treatment in order to determine the caspase-3 activity. DMSO served as the vehicle control. For compound names, see legend to Figure 1.

  4. Figure 4.
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    Figure 4.

    Effect of methyl antcinate A on apoptosis regulatory proteins. PC-3 cells were treated with 25 μM, 50 μM, and 100 μM of MAA or vehicle control (DMSO) for 6 h. Equal amounts of cell lysates were analyzed for Bcl-2, Bax, and PARP protein expression by western blotting with corresponding antibodies. β-Actin served as the loading control. The values are fold intensity of protein bands of MAA-treated PC-3 cells compared to the respective DMSO vehicle-treated control after being normalized to the respective internal reference (β-actin). The values in the third row denote the relative expression of full-length PARP but not cleaved PARP.

  • Copyright© 2012 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Anticancer Research: 32 (7)
Anticancer Research
Vol. 32, Issue 7
July 2012
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Anticancer Effects of Eleven Triterpenoids Derived from Antrodia camphorata
YI-PANG LEE, WAN-CHI TSAI, CHIH-JAN KO, YERRA KOTESWARA RAO, CHIUNG-RU YANG, DAR-REN CHEN, MING-HUI YANG, CHI-CHIANG YANG, YEW-MIN TZENG
Anticancer Research Jul 2012, 32 (7) 2727-2734;

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Anticancer Effects of Eleven Triterpenoids Derived from Antrodia camphorata
YI-PANG LEE, WAN-CHI TSAI, CHIH-JAN KO, YERRA KOTESWARA RAO, CHIUNG-RU YANG, DAR-REN CHEN, MING-HUI YANG, CHI-CHIANG YANG, YEW-MIN TZENG
Anticancer Research Jul 2012, 32 (7) 2727-2734;
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