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Research Article

Effects of (5Z)-7-Oxozeaenol on the Oxidative Pathway of Cancer Cells

ULYANA MUÑOZ ACUÑA, JENNIFER WITTWER, SLOAN AYERS, CEDRIC J. PEARCE, NICHOLAS H. OBERLIES and ESPERANZA J. CARCACHE DE BLANCO
Anticancer Research July 2012, 32 (7) 2665-2671;
ULYANA MUÑOZ ACUÑA
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JENNIFER WITTWER
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SLOAN AYERS
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CEDRIC J. PEARCE
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NICHOLAS H. OBERLIES
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ESPERANZA J. CARCACHE DE BLANCO
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  • For correspondence: carcache@pharmacy.ohio-state.edu
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Abstract

Aim: As part of an on going investigation of novel anticancer agents from natural origin, the biological and cellular effects of (5Z)-7-oxozeaenol on cancer cells were investigated. Materials and Methods: The expression of nuclear factor kappa B (NF-κB), IκB kinase (IKKα), IKKβ and caspase-3 were analyzed by western blot. Reactive oxygen species (ROS) fluorescence and caspase luminescent assays were used to assess the intracellular effects in HeLa cervical and HT-29 colon cancer cell lines. The mitochondrial transmembrane potential (MTP) was analyzed by fluorescence-activated cell sorting (FACS). Results: Cells treated with (5Z)-7-oxozeaenol exhibited down-regulation of NF-κB in a dose-dependent manner. Treatment with (5Z)-7-oxozeaenol significantly enhanced the levels of ROS in HeLa and HT-29 cells. MTP was reduced in HT-29 cells. The expression of caspase-3 and -7 was induced in (5Z)-7-oxozeaenol treated HeLa cells, in comparison with those treated with paclitaxel. Conclusion: Our findings suggest that (5Z)-7-oxozeaenol is a potent inhibitor of the NF-κB pathway and potentiates the production of ROS, as well as induces caspase-3 and -7 in HeLa and HT-29 cancer cells. Thus, (5Z)-7-oxozeaenol represents a new lead compound for drug development, particularly as a new cancer chemotherapeutic agent, since programmed cell death might be mediated through the activation of a caspase-arbitrated pathway.

  • (5Z)-7-Oxozeaenol
  • NF-κB pathway
  • apoptosis
  • caspase
  • IKKα
  • IKKβ
  • ROS
  • HeLa
  • HT-29 cells
  • Received May 8, 2012.
  • Revision received June 3, 2012.
  • Accepted June 5, 2012.
  • Copyright© 2012 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Anticancer Research: 32 (7)
Anticancer Research
Vol. 32, Issue 7
July 2012
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Effects of (5Z)-7-Oxozeaenol on the Oxidative Pathway of Cancer Cells
ULYANA MUÑOZ ACUÑA, JENNIFER WITTWER, SLOAN AYERS, CEDRIC J. PEARCE, NICHOLAS H. OBERLIES, ESPERANZA J. CARCACHE DE BLANCO
Anticancer Research Jul 2012, 32 (7) 2665-2671;

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Effects of (5Z)-7-Oxozeaenol on the Oxidative Pathway of Cancer Cells
ULYANA MUÑOZ ACUÑA, JENNIFER WITTWER, SLOAN AYERS, CEDRIC J. PEARCE, NICHOLAS H. OBERLIES, ESPERANZA J. CARCACHE DE BLANCO
Anticancer Research Jul 2012, 32 (7) 2665-2671;
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