Figure 6.
Delta-tocotrienol inhibits cell migration and invasion. Inhibition of NSCLC cell invasion by delta-tocotrienol, cisplatin, and their combination for 72 h. Upper panel: A549 (A) and H1650 (B) cells were seeded into Matrigel-coated inserts with delta-tocotrienol (15 μM), cisplatin (4 μM), the combination or DMSO (C). Cells that invaded to the lower surface of the insert over a period of 20 h were stained with crystal violet dye. Lower panel: The photometric intensity of the invaded cells was determined for the number of A549 and H1650 cells. Cell invasion is presented as means±S.E.M of three independent experiments. *p<0.05, **p<0.01. Inhibition of NSCLC cell migration by delta-tocotrienol (15 μM), cisplatin (4 μM), their combination or DMSO (C) using the wound healing assay. Uniform wounds were made by scratching the confluent cultures which were then treated with delta-tocotrienol (15 μM) alone, cisplatin (4 μM) alone, the combination of delta-tocotrienol (15 μM) and cisplatin (4 μM) or DMSO (C) for over 20 h. Then the wound healing images were captured using a microscope at 10× objective.