Figure 5.
Representative western blotting showing changes in the levels of apoptosis and endoplasmic reticulum (ER) stress-associated proteins in HL-60 cells after exposure to safrole. Cells were treated with 75 μM safrole for 0, 6, 12, 24, 48 and 72 h before total proteins were prepared and determined. The levels of apoptosis-related protein expressions were estimated by western blotting analysis, as described in the Materials and Methods. BCL-2 (B-cell lymphoma 2), BAX (BCL-2-associated X protein), APAF-1 (apoptotic protease activating factor-1), cytochrome c, AIF (apoptosis-inducing factor), XIAP (X-linked inhibitor of apoptosis protein), caspase-9, FAS (fatty acid synthase), caspase-3, caspase-8, PARP (poly (ADP-ribose) polymerase), GRP78 (glucose-regulated protein 78), GADD153 (growth arrest- and DNA damage-inducible gene 153) and ATF-4 (activating transcription factor-4) and ATF6-α.