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Research ArticleExperimental Studies

Quantification of Breast Cancer Cells in Peripheral Blood Samples by Real-Time RT-PCR

MICHAEL ZEBISCH, ALEXANDRA C. KÖLBL, CHRISTIAN SCHINDLBECK, JULIA NEUGEBAUER, SABINE HEUBLEIN, MATTHIAS ILMER, BRIGITTE RACK, KLAUS FRIESE, UDO JESCHKE and ULRICH ANDERGASSEN
Anticancer Research December 2012, 32 (12) 5387-5391;
MICHAEL ZEBISCH
1Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Munich, Germany
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ALEXANDRA C. KÖLBL
1Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Munich, Germany
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CHRISTIAN SCHINDLBECK
2Clinic Traunstein, Traunstein, Germany
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JULIA NEUGEBAUER
1Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Munich, Germany
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SABINE HEUBLEIN
1Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Munich, Germany
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MATTHIAS ILMER
3Department of Molecular Pathology, University of Texas, Houston, TX, U.S.A.
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BRIGITTE RACK
1Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Munich, Germany
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KLAUS FRIESE
1Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Munich, Germany
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UDO JESCHKE
1Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Munich, Germany
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  • For correspondence: udo.jeschke{at}med.uni-muenchen.de
ULRICH ANDERGASSEN
1Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Munich, Germany
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    Figure 1.

    Relative quantification (RQ) curves for cytokeratins 8, 18 and 19 in CAMA-1 cells. Quantification was relative to that of 18S.

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    Figure 2.

    Comparison of relative quantification (RQ) values of control samples (open symbols) vs. samples spiked with 10 CAMA-1 cells/1×106 blood cells (closed symbols).

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    Figure 3.

    Comparison of relative quantification (RQ) values of control samples (open symbols) vs. samples spiked with 10 MCF-7 cells/1×106 blood cells (closed symbols).

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    Figure 4.

    Comparison of relative quantification (RQ) values of control samples (open symbols) vs. samples spiked with 10 ZR-75-1 cells/1×106 blood cells (closed symbols).

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    Figure 5.

    Comparison of relative quantification (RQ) values of control samples (open symbols) vs. samples spiked with 10 cells of a mixture of the used cancer cell lines/1×106 blood cells (closed symbols).

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Anticancer Research
Vol. 32, Issue 12
December 2012
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Quantification of Breast Cancer Cells in Peripheral Blood Samples by Real-Time RT-PCR
MICHAEL ZEBISCH, ALEXANDRA C. KÖLBL, CHRISTIAN SCHINDLBECK, JULIA NEUGEBAUER, SABINE HEUBLEIN, MATTHIAS ILMER, BRIGITTE RACK, KLAUS FRIESE, UDO JESCHKE, ULRICH ANDERGASSEN
Anticancer Research Dec 2012, 32 (12) 5387-5391;

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Quantification of Breast Cancer Cells in Peripheral Blood Samples by Real-Time RT-PCR
MICHAEL ZEBISCH, ALEXANDRA C. KÖLBL, CHRISTIAN SCHINDLBECK, JULIA NEUGEBAUER, SABINE HEUBLEIN, MATTHIAS ILMER, BRIGITTE RACK, KLAUS FRIESE, UDO JESCHKE, ULRICH ANDERGASSEN
Anticancer Research Dec 2012, 32 (12) 5387-5391;
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Keywords

  • breast carcinoma
  • CTC
  • cytokeratin
  • MCF7
  • ZR-75-1 cells
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