Research ArticleExperimental Studies

Loss of Mesothelin Expression by Mesothelioma Cells Grown In Vitro Determines Sensitivity to Anti-Mesothelin Immunotoxin SS1P

JINGLI ZHANG, SHUO QIU, YUJIAN ZHANG, MARIA MERINO, PATRICIA FETSCH, ITZHAK AVITAL, ARMANDO FILIE, IRA PASTAN and RAFFIT HASSAN
Anticancer Research December 2012, 32 (12) 5151-5158;

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Figures

  • Figure 1.
    Figure 1.

    Characterization of patients' tissue biopsy and early-passage mesothelioma cells. H&E staining of the patients' original tissue biopsy (A) and mesothelin staining of original tissue biopsy using the anti-mesothelin mAb 5B2 (B). Morphological features of the sections made from the cell blocks of patient tumor cells grown in short-term culture (C) as well as mesothelin staining using the anti-mesothelin mAb 5B2 (D).

  • Figure 2.
    Figure 2.

    FACS analysis for mesothelin expression of patients' tumor cells grown in short-term culture. Cells were incubated with the anti-mesothelin mAb MN, conjugated with R-PE or isotype control antibody. Results are shown in terms of histogram plots for each cell line where the shaded area depicts the binding of MN antibody and the clear area shows the binding of isotype control antibody. The number of mesothelin sites/cell for each of the early-passage mesothelioma cell cultures is shown in each histograph.

  • Figure 3.
    Figure 3.

    Cytotoxicity of SS1P against early-passage mesothelioma cells. (A) NCI-M-02 cells (5×104) were seeded in a 24 well plate and treated with 10 ng/ml of SS1P or BL22. After 96 h of incubation the cells were washed, fixed and cell viability was assessed by the crystal violet assay. (B) Effect of SS1P (10 ng/ml) on the different early-passage mesothelioma cells using the crystal violet assay. The y-axis represents percent cell death as compared to control untreated cells. Data represents the mean±SD of triplicate samples. (C) Spearman correlation analysis was performed using Graphpad to show the correlation between SS1P cytotoxicity and mesothelin sites/cell expressed by the early-passage mesothelioma cells.

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Loss of Mesothelin Expression by Mesothelioma Cells Grown In Vitro Determines Sensitivity to Anti-Mesothelin Immunotoxin SS1P
JINGLI ZHANG, SHUO QIU, YUJIAN ZHANG, MARIA MERINO, PATRICIA FETSCH, ITZHAK AVITAL, ARMANDO FILIE, IRA PASTAN, RAFFIT HASSAN
Anticancer Research Dec 2012, 32 (12) 5151-5158;
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