Combination of Glucan, Resveratrol and Vitamin C Demonstrates Strong Anti-tumor Potential

Effects of two i.p. injections of glucan, vitamin C or resveratrol on formation of antibodies against ovalbumin. Mice were injected twice (two weeks apart) and the serum was collected 7 days after the last injection. The level of specific antibodies against ovalbumin was detected by ELISA. As positive control, Freund's adjuvant was used. *Represents significant differences between the control (ovalbumin alone) and samples at p≤0.05. Individual substances were used at 100 μg/dose. All experiments were performed in triplicate.

Therapy of Balb/c mice with Ptas64 mammary carcinoma. Data from three independent experiments are shown. For each experiment, groups of mice were tested for a response to a therapy as indicated by the weight of tumors after two weeks of therapy. For each experiment, individual groups were given daily ip. injections of 100 μg of glucan, resveratrol, vitamin C, or their combination. The control group (659.7±38.5 [mg]) of mice received daily ip. PBS. Each value represents the mean±SD. *Represents significant differences between the control and samples at p≤0.05 level. All experiments were performed in triplicate.

Effect of tested substances on lung cancer growth in cyclophosphamide (CY)-treated mice. CY (30 mg/kg) was injected into mice on day 8 of the inoculation of 1×10⁵ tumor cells. Other experimental groups obtained the daily injections of individual substances over 5 days starting 48 h after injection of CY. Individual substances were used at 100 μg/dose. Each value represents the mean±SD. *Represents significant differences between the control and samples at p≤0.05 level. All experiments were performed in triplicate.