Article Figures & Data
Figures
- Figure 1.
Chemical structure of glycyrrhizin, 18β-glycyrrhetinic acid and glabridin.
- Figure 2.
Cell viability assay (MTT) of keratinocyte cultures after glycyrrhizin (A), 18β-glycyrrhetinic acid (B) or glabridin (C) treatment. *p≤0.01 and **p≤0.001 by t-test with respect to control (100% growth).
- Figure 3.
Comet assay of UVB-irradiated keratinocytes in the absence or presence of glycyrrhizin (GL), 18β-glycyrrhetinic acid (18β-GA) or glabridin (GLB). Images from epifluorescence microscopy. UVB treatment: 50 mJ/cm2, 75 mJ/cm2, 100 mJ/cm2. Untreated cells (control): fluorescence confined to the nucleus. DNA fragmentation: fragments migrate from the nucleus in typical comet tails.
- Figure 4.
ROS assay. (A) Human keratinocytes treated for 24 hours. (B) Human keratinocytes irradiated (UVB 50 mJ/cm2, 75 mJ/cm2 or 100 mJ/cm2) for 24 hours and treated with glycyrrhizin 30 μM, 18β-glycyrrhetinic acid 30 μM or glabridin 15 μM. ROS production expressed as intracellular antioxidant activity. *p≤0.01, **p≤0.001 by t-test with respect to control.
- Figure 5.
Effect of GL 30 μM, 18β-GA 30 μM and GLB 15 μM on p53 (A) and bcl-2 (B) expression, by Western blot analysis. Protein levels were determined in NHK in the presence or absence of three different UVB doses. *p≤0.01; **p≤0.001 by t-test with respect to UVB-irradiated control.
- Figure 6.
Western blot analysis. PARP cleavage was compared in treated and non-treated NHKs (glycyrrhizin, GL, 30 μM; 18β-glycyrrhetinic acid 18β-GA 30 μM or glabridin, GLB, 15 μM) in the presence or absence of three different UVB doses.
