Figure 3.
Effects of the synthetic cannabinoid WIN-55,212 (WIN) and cannabidiol (CBD) on phosphatase mRNA levels in LNCaP cells. Induction by WIN (A, B) and CBD (C, D). LNCaP cells were treated with dimethylsulfoxide (DMSO), 7.5 μM WIN and 15 μM CBD for 24 h, and dual specificity phosphatase 1 (DUSP1), dual specificity phosphatase 4 (DUSP4), dual specificity phosphatase 10 (DUSP10), serum prostatic acid phosphate (sACPP), cellular prostatic acid phosphate (cACPP), protein tyrosine phosphatase, non-receptor type 1 (PTPN1), protein phosphatase 2A activator, regulatory subunit 4 (PPP2R4), protein tyrosine phosphatase, receptor type, J (PTPRJ) and protein tyrosine phosphatase, non-receptor type 6 (PTPN6) mRNA levels were determined by real-time PCR as described in the Materials and Methods. Results are expressed as mean±S.E. for 3 replicate experiments and significant (p<0.05) increases are indicated (*).