Article Figures & Data
Figures
- Figure 1.
A: Schematic diagram of the sonication setup. B: The intensity distribution of pulsed ultrasound. The measured plane in the sonication tank was 8 cm away from the transducer, where the cell suspension bags were located.
- Figure 2.
A: The effects of ALA solution of different concentrations on SAS cells growth after different incubation periods (n=3). B: Survival rates of SAS cells after sonication alone for 1, 2, 3, 4 and 5 min (n=6). C: Survival rates of SAS cells after 2 min sonication plus ALA at 0, 1, 10 and 50 μg/ml (n=6). Data are presented as the mean±standard deviation.
- Figure 3.
Apoptosis rates of SAS cells after treatment (n=6). Data are presented as the mean±standard deviation. C: Control group without treatment; A: 10 μg/ml ALA alone; U: 2 min sonication alone; UA: 2 min sonication plus 10 μg/ml ALA; *p<0.05 compared to C; †p<0.05 compared to U.
- Figure 4.
Intracellular reactive oxygen species (ROS) production in SAS cells (n=6). Data are presented as the mean±standard deviation. C: Control group without treatment; A: 10 μg/ml ALA alone; U: 2 min sonication alone; UA: 2 min sonication plus 10 μg/ml ALA; *p<0.05 compared to C; †p <0.05 compared to U.
- Figure 5.
Lipid peroxidation levels of SAS cells after treatment (n=6). Data are presented as the mean±standard deviation. C: Control group without treatment; A: 10 μg/ml ALA alone; U: 2 min sonication alone; UA: 2 min sonication plus 10 μg/ml ALA; *p<0.05 compared to C; †p<0.05 compared to U.
- Figure 6.
MMP decrease of SAS cells after treatment. The histograms representing cell counts versus fluorescence intensity of rhodamine123 are representative of four independent experiments. M1: Cells with loss of MMP. A: control group without treatment; B: 10 μg/ml ALA alone; C: 2 min sonication alone; D: 2 min sonication plus 10 μg/ml ALA.
