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Research ArticleExperimental Studies

Inhibition of Growth and Induction of Alkaline Phosphatase in Colon Cancer Cells by Flavonols and Flavonol Glycosides

MICHAEL A. LEA, CHINWE IBEH, JILL K. DEUTSCH, IMRAN HAMID and CHARLES desBORDES
Anticancer Research September 2010, 30 (9) 3629-3635;
MICHAEL A. LEA
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  • For correspondence: lea{at}umdnj.edu
CHINWE IBEH
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JILL K. DEUTSCH
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IMRAN HAMID
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CHARLES desBORDES
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    Figure 1.

    Effects of incubation of Caco-2 cells for 72 hours with 25 μM quercetin, quercetin 3-glucoside (Q3G) and rutin on enzyme activities. The data are given as the means and standard deviations for three determinations. Relative to control activities, there were significant increases in alkaline phosphatase (A) and dipeptidyl peptidase (B) after incubation with quercetin and quercetin 3-glucoside (p<0.05) but not with rutin.

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    Figure 2.

    Inhibition of the incorporation of [3H]thymidine incorporation into DNA in SW1116 cells (A) and NCM460 cells (B). Incubations for 72 hours were with flavonol concentrations of 50 μM (A) or 25 μM (B). The data are expressed as means and standard deviations for six determinations.

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    Figure 3.

    Inhibition of the incorporation of [3H]thymidine incorporation into DNA in Caco-2 cells after a 72 hour incubation with quercetin 3-glucoside. The data are expressed as means and standard deviations for six determinations.

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    Figure 4.

    Effects of incubation of Caco-2 cells for 72 hours with 0.5 mM butyrate and 10 μM quercetin 3-glucoside (Q3G) as single agents and in combination on alkaline phosphatase and dipeptidyl peptidase activities. The data are expressed as the means and standard deviations for three determinations. Relative to controls, there were statistically significant increases in alkaline phosphatase activity with all treatments and for dipeptidyl peptidase activity with the combined treatment (p<0.05).

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    Figure 5.

    Effects of incubation of Caco-2 cells for 72 hours with 0.5 mM butyrate and 50 μM myricetin (myric.) in Figure 5A and 25 μM quercetin 4′-glucoside (Q4'G) in Figure 5B as single agents and in combination on activity of alkaline phosphatase. The data are expressed as the means and standard deviations for three determinations. The activity with the combined treatments was greater than with either single agent (p<0.05).

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    Figure 6.

    Assay of hydrogen peroxide formation after incubation of flavonols with RPMI-1640 medium containing 5% fetal calf serum. Quercetin 3-rhamnoside is described as quercitrin. The results represent the means of duplicate assays in two separate experiments.

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    Figure 7.

    Effects of incubation of Caco-2 cells for 72 hours with 25 μM apigenin, baicalein and quercetin on alkaline phosphatase activity. The data are given as the means and standard deviations for three determinations.

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    Figure 8.

    Effects of incubation of Caco-2 cells for 72 hours with increasing concentrations of hydrogen peroxide on alkaline phosphatase activity. The data are given as the means and standard deviations for three determinations.

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    Figure 9.

    Effects of incubation of Caco-2 cells for 72 hours with 100 units catalase/ml (catal.) and 50 μM baicalein (baical.) as single agents and in combination on alkaline phosphatase activity. The agents were added at the initiation of the 72-hour incubation. The data are expressed as the means and standard deviations for three determinations. The activity with baicalein as a single agent was significantly greater than in controls (p<0.05) but was not significantly different from activity observed in combination with catalase.

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Anticancer Research
Vol. 30, Issue 9
September 2010
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Inhibition of Growth and Induction of Alkaline Phosphatase in Colon Cancer Cells by Flavonols and Flavonol Glycosides
MICHAEL A. LEA, CHINWE IBEH, JILL K. DEUTSCH, IMRAN HAMID, CHARLES desBORDES
Anticancer Research Sep 2010, 30 (9) 3629-3635;

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Inhibition of Growth and Induction of Alkaline Phosphatase in Colon Cancer Cells by Flavonols and Flavonol Glycosides
MICHAEL A. LEA, CHINWE IBEH, JILL K. DEUTSCH, IMRAN HAMID, CHARLES desBORDES
Anticancer Research Sep 2010, 30 (9) 3629-3635;
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