Figure 5.
Time-dependent effect of MβCD treatment on endocytic activity and viability of J774 cells. J774 cells (5×105 cells/ml) were incubated with 2.5 mM MβCD for 30 min, and then F-PSL MS were added to give a microshere: macrophage ratio of 10, followed by incubation for different periods of time. The endocytic activity and viability of J774 cells were determined as described in the Materials and Methods. Note that the incubation period in the shown is that for F-PSL MS, and hence the total incubation period with MβCD is 30 minutes longer than that shown in the figures. A: Endocytic activity of F-PSL MS by J774 cells. B: Viability of J774 cells relative to the value after incubation for 1 hour without treatment with MβCD. In the case of treatment without MβCD, J774 cells were first incubated in the incubation medium for 30 minutes, and then F-PSL MS were added. Results are expressed as the mean±SD (n=9). Statistically significant differences at the level of **p<0.01 and ***p<0.001, from the control (untreated cells), as assessed by Student's t-test, are shown.