Article Figures & Data
Figures
- Figure 1.
Structure of epigallocatechin-3-gallate (EGCG).
- Figure 2.
Effect of EGCG on SKOV-3 cell viability. After 2 days of treatment, cell viability was determined by MTS assay. *p<0.01 Compared to control.
- Figure 3.
Effect of EGCG on SKOV-3 cell proliferation. After 2 days of treatment, DNA synthesis was measured by BrdU incorporation assay. *p<0.01 Compared to control.
- Figure 4.
Effect of EGCG on cell cycle progression. After 2 days of treatment, the cell cycle phase distribution was determined by FACS analysis.
- Figure 5.
Effect of EGCG on cellular morphology of SKOV-3 cells. The cells were treated with EGCG for 2 days. A: Cells treated with vehicle (PBS) control; B-F: cells treated with EGCG at B: 10 μg/ml; C: 20 μg/ml; D: 40 μg/mL; E: 60 μg/ml; and F: 80 μg/ml EGCG. The arrows indicate cell death characterized by cellular shrinkage.
- Figure 6.
DNA fragmentation analysis of SKOV-3 cells treated with EGCG (50 μg/m) for 2 days. Lane 1: Positive control (apoptotic U937 cells supplied with the DNA ladder kit); lane 2: DNA standard; lane 3: vehicle-treated cells; lane 4: EGCG-treated cells.
- Figure 7.
TUNNEL assay of SKOV-3 cells treated with EGCG (30 μg/ml) for 2 days. Representative photomicrographs of overlaid apoptotic stain (green) and nuclear stain (red). TUNEL-positive nuclei due to DNA fragmentation (bright yellow spots) are indicated by arrows. A: control; B: EGCG-treated cells.
