Research ArticleExperimental Studies

Antitumor Potential of Three Herbal Extracts against Human Oral Squamous Cell Lines

QING CHU, KAZUE SATOH, TAISEI KANAMOTO, SHIGEMI TERAKUBO, HIDEKI NAKASHIMA, QINTAO WANG and HIROSHI SAKAGAMI
Anticancer Research August 2009, 29 (8) 3211-3219;

Article Figures & Data

Figures

  • Figure 1.
    Figure 1.

    Effect of sodium ascorbate and mitoxantrone on DNA fragmentation of human OSCC (HSC-2, NA) and promyelocytic leukemia HL-60 cell lines. Cells were incubated for 6, 24 or 48 hours with the indicated concentrations of sodium ascorbate (VC) or mitoxantrone (MITO), and processed for DNA fragmentation (A) and caspase activation (B).

  • Figure 2.
    Figure 2.

    Combination effect of three herbal extracts (DB, AS and CO) and sodium ascorbate. Near confluent HSC-2 cells were incubated for 48 hours without (control) or with the indicated concentrations of DB, AS or CO, in combination with sodium ascorbate (VC), and the viable cell number was then determined by MTT method. Each value represents the mean from triplicate assays.

  • Figure 3.
    Figure 3.

    Combination effect of three herbal extracts (DB, AS and CO) and mitoxantrone. Near confluent HSC-2 cells were incubated for 48 hours without (control) or with the indicated concentrations of DB, AS or CO, in combination with mitoxantrone (MITO), and the viable cell number was determined by MTT method. Each value represents the mean from triplicate assays.

  • Figure 4.
    Figure 4.

    Radical production by herbal extracts. Samples (20 mg/ml) were mixed with an equal volume of 0.2 M buffer solution at the indicated pH, and the radical intensity was then measured by ESR. DB and CO produced a broad radical peak (indicated by arrow) under alkaline conditions, and its radical intensity increased with increasing pH, whereas AS did not produce any detectable radical intensity.

  • Figure 5.
    Figure 5.

    ESR spectra of DMPO-OOH adduct produced in the HX-XOD reaction mixture with the indicated concentration of CO. When the superoxide anion was reacted with DMPO, four radical peaks (arrows) of the spin adduct (DMPO-OOH) were generated. The height of the superoxide anion peak was diminished in the presence of increasing concentrations of CO.

  • Figure 6.
    Figure 6.

    Superoxide anion and hydroxyl radical scavenging activity of three herbal extracts (DB, AS and CO). Radical intensity of the first peak of DMPO-OOH (produced from HX-XOD reaction) (A) and DMPO-OH radical (produced by Fenton reaction) (B) in the presence of increasing concentrations of each extract was shown. Each value represents the mean±S.D. from triplicate assays.

Tables

  • Table I.
  • Table II.
  • Table III.
  • Table IV.
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Antitumor Potential of Three Herbal Extracts against Human Oral Squamous Cell Lines
QING CHU, KAZUE SATOH, TAISEI KANAMOTO, SHIGEMI TERAKUBO, HIDEKI NAKASHIMA, QINTAO WANG, HIROSHI SAKAGAMI
Anticancer Research Aug 2009, 29 (8) 3211-3219;
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