Article Figures & Data
Figures
- Figure 1.
Structures of tested compounds.
- Figure 2.
The effect of DOX and its formamidinoderivatives DOXM and DOXH on DNA cleavage by topoisomerase II. Representative images of agarose gels are shown. Cleavage assay was performed in the absence or presence of drugs as described under Materials and Methods. A, Effect of DOX: lane 1, control (reaction mixture carried out without drug); lanes 2-4, reaction in the presence of 5, 10 and 25 μM; DOX; lane 5, 100 μM etoposide. B, Effect of DOXM: lane 6, control; lanes 7-10, reaction in the presence of 5, 10, 25 and 50 μM DOXM. C, Effect of DOXH: lane 11, control; lanes 12-15, reaction in the presence of 5, 10, 25 and 50 μM DOXH. N, nicked DNA; L, linear DNA; S, supercoiled DNA. Under these conditions of electrophoresis, relaxed DNA migrates along with supercoiled DNA and these forms cannot be distinguished.
- Figure 3.
Stimulation of supercoiled pBR322 DNA cleavage by tested compounds. The amount of linear DNA was estimated by gel densitometric measurements. Data are means of three independent experiments±SD. *Amount of linear DNA formed in the presence of drug was significantly higher than that in the control assay, p<0.05.
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