Research ArticleExperimental Studies

siRNA Targeting HIF-1α Induces Apoptosis of Pancreatic Cancer Cells through NF-κB-independent and -dependent Pathways under Hypoxic Conditions

CHUANGUI CHEN and ZHENTAO YU
Anticancer Research April 2009, 29 (4) 1367-1372;

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  • Figure 1.
    Figure 1.

    Expression of HIF-1α mRNA and protein after 24 h and 48 h under hypoxic conditions. (A) Expression of HIF-1α mRNA determined by real-time PCR. (B) Expression of HIF-1α protein determined by western blot. Western blot of HIF-1α protein expression (C) after 24 h and (D) after 48 h. Wt: non-transfected wild-type MiaPaCa2 cells; Cv: MiaPaCa2 cells transfected with rAAV-hrGFP; Si: MiaPaCa2 cells transfected with rAAV-siHIF.

  • Figure 2.
    Figure 2.

    The activity of NF-κB in MiaPaCa2 cells determined by EMSA under hypoxic conditions. (A) From gel electrophoresis analysis, the NF-κB complex had two bands, p50/p50 and p50/p65. (B) Relative NF-κB activity. Wt: non-transfected wild-type MiaPaCa2 cells; Cv: MiaPaCa2 cells transfected with rAAV-hrGFP; Si: MiaPaCa2 cells transfected with rAAV-siHIF.

  • Figure 3.
    Figure 3.

    (A) Apoptosis of MiaPaCa2 cells without or with PDTC under hypoxic conditions determined by TUNEL assay. (B) Proliferation of MiaPaCa2 cells without or with PDTC under hypoxic conditions determined by MTT. Wt: non-transfected wild-type MiaPaCa2 cells; Cv: MiaPaCa2 cells transfected with rAAV-hrGFP; Si: MiaPaCa2 cells transfected with rAAV-siHIF.

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siRNA Targeting HIF-1α Induces Apoptosis of Pancreatic Cancer Cells through NF-κB-independent and -dependent Pathways under Hypoxic Conditions
CHUANGUI CHEN, ZHENTAO YU
Anticancer Research Apr 2009, 29 (4) 1367-1372;
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