Figure 3.
Activation of caspases and alteration of caspase target molecules by the combined treatment of sanguinarine and TRAIL in TRAIL-resistant AGS cells. AGS cells were treated with the indicated concentrations of sanguinarine and/or TRAIL for 24 h. A, Equal amounts of cell lysate (20 μg/μl) were resolved by SDS-polyacryamide gel electrophoresis, transferred to nitrocellulose membranes, and probed with anti-caspase-3, anti-caspase-8, anti-caspase-9, anti-PARP, anti-β-catenin, and anti-actin antibodies. Actin was used as the internal control. B, Caspase-3, -8, and -9 activities were determined following the manufacturer's protocol. Each point represents the mean±SD of three independent experiments. The significance was determined by Student's t-test (*p<0.05 versus untreated control).