Article Figures & Data
Figures
- Figure 1.
Effect of Thymax (Thy) on percent of DC activation: co-stimulatory and maturation markers CD40, CD80, CD83 and CD86. Monocyte-derived DCs were treated with Thymax (10 or 20 μg/ml) or LPS (1 μg/ml, control) for 24 h. Expression of cell surface markers was determined by flow cytometry and is shown as the mean fluorescent intensity (MFI). One representative donor is shown, and the data show the mean±SD from 3 individual experiments;* p<0.05; as compared to control LPS-treated cells.
- Figure 2.
Effect of Thymax on cytokine production: the levels of IL-6, IL-10 and IL-12p40 secreted by the DCs. Immature DCs were incubated with LPS (1 μg/ml) or 10 or 20 μg/ml Thymax for 24 h. Supernatants were collected and IL-6, IL-10 and IL-12p40 were measured by specific ELISA kits. One representative donor is shown, and the data are the mean±SD from 3 individual experiments; *p<0.05 as compared to control LPS-treated cells.
- Figure 3.
Effect of Thymax on the activation of T-cell proliferation: CD8+ T-cells and CD4+ T-cells. DCs were stimulated with LPS (1 μg/ml) or Thymax (10 or 20 μg/ml) for 24 h. After washing, DCs were cultured with CFSE-labeled T-cells for 5 days. Proliferation of T-cells was measured by dilution of CFSE dye. One representative donor is shown from 1 of 3 individual experiments.
- Figure 4.
IFN-γ secretion in Thymax primed DC-induced T-cells. DCs were stimulated with LPS (1 μg/ml) or Thymax (10 or 20 μg/ml) for 24 h. After washing, DCs were cultured with CFSE-labeled T-cells for 5 days. The secretion of IFN-γ in the supernatants was assessed using specific ELISA. One representative donor is shown, and the data are the mean±SD from 3 individual experiments; *p<0.05, as compared to control LPS treated cells.
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