Skip to main content

Main menu

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Subscribers
    • Advertisers
    • Editorial Board
    • Special Issues 2025
  • Journal Metrics
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Anticancer Research
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Anticancer Research

Advanced Search

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Subscribers
    • Advertisers
    • Editorial Board
    • Special Issues 2025
  • Journal Metrics
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Visit us on Facebook
  • Follow us on Linkedin
Research ArticleExperimental Studies

Multitarget siRNA Inhibition of Antiapoptotic Genes (XIAP, BCL2, BCL-XL) in Bladder Cancer Cells

DOREEN KUNZE, DANIELA WUTTIG, SUSANNE FUESSEL, KAI KRAEMER, MATTHIAS KOTZSCH, AXEL MEYE, MARC-OLIVER GRIMM, OLIVER W. HAKENBERG and MANFRED P. WIRTH
Anticancer Research July 2008, 28 (4B) 2259-2263;
DOREEN KUNZE
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: doreen.kunze@uniklinikum-dresden.de
DANIELA WUTTIG
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
SUSANNE FUESSEL
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
KAI KRAEMER
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
MATTHIAS KOTZSCH
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
AXEL MEYE
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
MARC-OLIVER GRIMM
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
OLIVER W. HAKENBERG
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
MANFRED P. WIRTH
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Background: The knockdown of XIAP, BCL2 and BCL-XL by siRNAs represents a promising treatment option for bladder cancer (BCa) since the overexpression of antiapoptotic genes is often associated with tumor progression and treatment resistance. Materials and Methods: EJ28 BCa cells were transfected with siRNAs - separately and combined - followed by analysis of target expression, viability, clonogenic survival, apoptosis and cell cycle. Furthermore, a possible chemosensitization by siRNA pretreatment was investigated. Results: The siRNA-mediated inhibition of these targets - either separately or combined - reduced the targets' expression, reduced cell growth and sensitized cells to a subsequent chemotherapy. Conclusion: Since tumor cells may bypass the inhibition of a single gene by changing their expression profile, e.g. switch from BCL2 to BCL-XL, the combined knockdown of multiple genes of the same pathway might be more effective in killing cancer cells. The siRNAs used represent appropriate tools for this aim since they reduced their targets' expression significantly and long-lastingly.

  • BCL2
  • BCL2L1
  • BIRC4
  • siRNA
  • chemosensitization
  • inhibition of apoptosis

Footnotes

  • ↵* Both authors contributed equally to this work.

  • Received January 23, 2008.
  • Revision received April 4, 2008.
  • Accepted April 21, 2008.
  • Copyright© 2008 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
PreviousNext
Back to top

In this issue

Anticancer Research: 28 (4B)
Anticancer Research
Vol. 28, Issue 4B
July-August 2008
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Anticancer Research.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Multitarget siRNA Inhibition of Antiapoptotic Genes (XIAP, BCL2, BCL-XL) in Bladder Cancer Cells
(Your Name) has sent you a message from Anticancer Research
(Your Name) thought you would like to see the Anticancer Research web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
2 + 2 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
Multitarget siRNA Inhibition of Antiapoptotic Genes (XIAP, BCL2, BCL-XL) in Bladder Cancer Cells
DOREEN KUNZE, DANIELA WUTTIG, SUSANNE FUESSEL, KAI KRAEMER, MATTHIAS KOTZSCH, AXEL MEYE, MARC-OLIVER GRIMM, OLIVER W. HAKENBERG, MANFRED P. WIRTH
Anticancer Research Jul 2008, 28 (4B) 2259-2263;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
Multitarget siRNA Inhibition of Antiapoptotic Genes (XIAP, BCL2, BCL-XL) in Bladder Cancer Cells
DOREEN KUNZE, DANIELA WUTTIG, SUSANNE FUESSEL, KAI KRAEMER, MATTHIAS KOTZSCH, AXEL MEYE, MARC-OLIVER GRIMM, OLIVER W. HAKENBERG, MANFRED P. WIRTH
Anticancer Research Jul 2008, 28 (4B) 2259-2263;
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • siRNA-mediated Inhibition of Antiapoptotic Genes Enhances Chemotherapy Efficacy in Bladder Cancer Cells
  • Google Scholar

More in this TOC Section

  • Role of the Circadian Clock Gene DEC1 in Chemosensitivity and Apoptosis in Colorectal Cancer
  • Selective Synergy of Ivermectin Combined With Recombinant Methioninase Against Colon-Cancer Cells in Contrast to Normal Fibroblasts
  • Trametinib Synergized With Abemaciclib to Inhibit Tumor Growth in Human Head and Neck Squamous Cell Carcinoma Cells
Show more Experimental Studies

Similar Articles

Anticancer Research

© 2025 Anticancer Research

Powered by HighWire