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Research ArticleExperimental Studies

Comparative Analysis of Cell Death Induction by Cisplatin and 5-FU in Human Oral Squamous and Hepatocellular Carcinoma Cell Lines

MASAHIKO OKAMURA, JUN SHIMADA and HIROSHI SAKAGAMI
Anticancer Research January 2008, 28 (1A) 253-259;
MASAHIKO OKAMURA
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JUN SHIMADA
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HIROSHI SAKAGAMI
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  • For correspondence: sakagami@dent.meikai.ac.jp
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Abstract

We established the optimal conditions for the induction of cell death by cisplatin (CDDP) and 5-fluorouracil (5-FU) in human oral squamous cell carcinoma (HSC-2, HSC-3, HSC-4) and human hepatocellular carcinoma (HepG2) cell lines. HSC-3 cells were the most sensitive to 48 hours' continuous treatment with CDDP, followed by HepG2, HSC-2 and HSC-4 cells. On the other hand, HSC-4 cells were the most sensitive to 48-hour continuous treatment with 5-FU, followed by HSC-2, HSC-3 and HepG2 cells. CDDP induced internucleosomal DNA fragmentation in HSC-2 and HSC-3 cells, but not in HSC-4 cells, while 5-FU failed to induce internucleosomal DNA fragmentation in all of these cells. The treatment of HSC-2, HSC-3 and HSC-4cells with CDDP for 12 hours (followed by incubation for 36 hours without CDDP) showed comparable magnitude of cytotoxicity and caspase-3 activation with that attained by continuous 48-hour CDDP treatment. On the other hand, the cytotoxicity of 5-FU depended both on the dose and the exposure time. The present study demonstrate that the most effective treatment time is 12 hours for CDDP and much longer for 5-FU in all studied cell lines, underlining the importance of optimizing the treatment time for each chemotherapeutic agent.

  • Cisplatin
  • 5-fluorouracil
  • chemotherapy
  • oral squamous cell carcinoma
  • Received September 18, 2007.
  • Revision received November 15, 2007.
  • Accepted December 11, 2007.
  • Copyright© 2008 International Institute of Anticaner Research (Dr. John G. Delinassios), All rights reserved
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Anticancer Research: 28 (1A)
Anticancer Research
Vol. 28, Issue 1A
January-February 2008
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Comparative Analysis of Cell Death Induction by Cisplatin and 5-FU in Human Oral Squamous and Hepatocellular Carcinoma Cell Lines
MASAHIKO OKAMURA, JUN SHIMADA, HIROSHI SAKAGAMI
Anticancer Research Jan 2008, 28 (1A) 253-259;

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Comparative Analysis of Cell Death Induction by Cisplatin and 5-FU in Human Oral Squamous and Hepatocellular Carcinoma Cell Lines
MASAHIKO OKAMURA, JUN SHIMADA, HIROSHI SAKAGAMI
Anticancer Research Jan 2008, 28 (1A) 253-259;
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