Facilitation of H₂O₂-induced A172 Human Glioblastoma Cell Death by Insertion of Oxidative Stress-sensitive TRPM2 Channels

Abstract

The melastatin-like transient receptor potential M2 (TRPM2) channel is a Ca²⁺ permeable channel that is activated by reactive oxygen species (ROS), and its activation induces necrotic cell death. The effect of insertion of TRPM2 into A172 human glioblastoma cells (A172 cells) was investigated. The insertion of TRPM2 channels enhanced cell death induced by H₂O₂ in the A172 cells. An H₂O₂-induced Ca²⁺ increase was observed in TRPM2-expressing cells, but not in wild-type cells. Proliferation, migration and invasion activities were not affected by the expression of TRPM2. TRPM2 seems to be a candidate for gene therapy in glioblastoma cells, since the insertion of TRPM2 into A172 cells can facilitate cell death through Ca²⁺ increase after H₂O₂ treatment without increasing malignancy.