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Research ArticleClinical Studies

Biochemical Nature and Mapping of PSMA Epitopes Recognized by Human Antibodies Induced after Immunization with Gene-based Vaccines

K. TODOROVA, S. ZOUBAK, M. MINCHEFF and S. KYURKCHIEV
Anticancer Research November 2005, 25 (6C) 4727-4732;
K. TODOROVA
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S. ZOUBAK
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M. MINCHEFF
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S. KYURKCHIEV
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  • For correspondence: kyurkch@hotmail.com
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Abstract

Background: A possible new target for immunotherapy is the prostate-specific membrane antigen (PSMA). The aim of the present study was to define potential PSMA epitopes for antibody binding using sera from patients immunized with gene-based anti-PSMA vaccines. Materials and Methods: Sera from prostate cancer patients, immunized repeatedly with plasmid and adenoviral vectors, each encoding for the extracellular portion of human PSMA, were tested for anti-PSMA antibodies by Western blot. PSMA-producing LNCaP cells were used as a control. Recombinant PSMA protein cleaved with different proteinases was used for epitope mapping. Different enzymes were used to cleave the PSMA molecule. Results: Specific anti-PSMA antibodies were detected in the studied patients' sera, mainly against the PSMA protein core. An alignment of the predicted enzyme-cleavage fragments was compared with Western blot results and several antibody epitopes were determined. Conclusion: These data demonstrate that multiple gene-based vaccinations induce an anti-PSMA humoral immune response. The antibodies are predominantly specific for the PSMA protein core.

  • Prostate cancer
  • gene-based PSMA vaccination
  • LNCaP cells
  • anti-PSMA antibodies
  • carbohydrate cleaving enzyme
  • proteases

Footnotes

  • Received May 23, 2005.
  • Accepted August 30, 2005.
  • Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Anticancer Research: 25 (6C)
Anticancer Research
Vol. 25, Issue 6C
1 Nov 2005
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Biochemical Nature and Mapping of PSMA Epitopes Recognized by Human Antibodies Induced after Immunization with Gene-based Vaccines
K. TODOROVA, S. ZOUBAK, M. MINCHEFF, S. KYURKCHIEV
Anticancer Research Nov 2005, 25 (6C) 4727-4732;

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Biochemical Nature and Mapping of PSMA Epitopes Recognized by Human Antibodies Induced after Immunization with Gene-based Vaccines
K. TODOROVA, S. ZOUBAK, M. MINCHEFF, S. KYURKCHIEV
Anticancer Research Nov 2005, 25 (6C) 4727-4732;
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