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Research ArticleExperimental Studies

Effects of Heavy Water (D2O) on Human Pancreatic Tumor Cells

JOHANNES HARTMANN, YVONNE BADER, ZSUZSANNA HORVATH, PHILIPP SAIKO, MICHAEL GRUSCH, CHRISTOPH ILLMER, SIBYLLE MADLENER, MONIKA FRITZER-SZEKERES, NICOLE HELLER, RUDOLF-GIESBERT ALKEN and THOMAS SZEKERES
Anticancer Research September 2005, 25 (5) 3407-3411;
JOHANNES HARTMANN
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YVONNE BADER
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ZSUZSANNA HORVATH
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PHILIPP SAIKO
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MICHAEL GRUSCH
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CHRISTOPH ILLMER
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SIBYLLE MADLENER
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MONIKA FRITZER-SZEKERES
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NICOLE HELLER
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RUDOLF-GIESBERT ALKEN
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THOMAS SZEKERES
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  • For correspondence: thomas.szekeres@meduniwien.ac.at
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Abstract

Background: Pancreatic cancer constitutes an entity which is difficult to treat and, therefore, mostly fatal. Since heavy water (deuterium oxide, D2O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its effects in human pancreatic tumor cells. Materials and Methods: The cytotoxic effects of D2O were examined in three pancreatic cancer cell lines (AsPC-1, BxPC-3 and PANC-1). Induction of apoptosis was determined by Hoechst/propidium iodide double staining and cell cycle distribution was investigated by FACS analysis. Results: Employing a clonogenic assay, D2O yielded IC50 values of 15%, 18% and 27% in AsPC-1, PANC-1 and BxPC-3 cells, respectively, and led to the induction of apoptosis when compared to untreated controls. Moreover, D2O caused a cell cycle arrest in the G2-M-phase (BxPC-3, PANC-1) or in the S-phase (AsPC-1). Conclusion: It is hoped that D2O might offer an additional option for the treatment of pancreatic carcinomas.

  • Heavy water (deuterium oxide, D2O)
  • apoptosis
  • cell cycle distribution
  • pancreatic cancer
  • AsPC-1
  • BxPC-3 and PANC-1 cells

Footnotes

  • Received March 18, 2005.
  • Accepted May 23, 2005.
  • Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Anticancer Research: 25 (5)
Anticancer Research
Vol. 25, Issue 5
1 Sep 2005
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Effects of Heavy Water (D2O) on Human Pancreatic Tumor Cells
JOHANNES HARTMANN, YVONNE BADER, ZSUZSANNA HORVATH, PHILIPP SAIKO, MICHAEL GRUSCH, CHRISTOPH ILLMER, SIBYLLE MADLENER, MONIKA FRITZER-SZEKERES, NICOLE HELLER, RUDOLF-GIESBERT ALKEN, THOMAS SZEKERES
Anticancer Research Sep 2005, 25 (5) 3407-3411;

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Effects of Heavy Water (D2O) on Human Pancreatic Tumor Cells
JOHANNES HARTMANN, YVONNE BADER, ZSUZSANNA HORVATH, PHILIPP SAIKO, MICHAEL GRUSCH, CHRISTOPH ILLMER, SIBYLLE MADLENER, MONIKA FRITZER-SZEKERES, NICOLE HELLER, RUDOLF-GIESBERT ALKEN, THOMAS SZEKERES
Anticancer Research Sep 2005, 25 (5) 3407-3411;
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