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Research ArticleClinical Studies

Molecular Staging Using Qualitative RT-PCR Analysis Detecting Thyreoglobulin mRNA in the Peripheral Blood of Patients with Differentiated Thyroid Cancer After Therapy

NIKI KARAVITAKI, PETER LEMBESSIS, MARINELA TZANELA, VARVARA VLASSOPOULOU, NICHOLAOS THALASSINOS and MICHAEL KOUTSILIERIS
Anticancer Research July 2005, 25 (4) 3135-3142;
NIKI KARAVITAKI
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PETER LEMBESSIS
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MARINELA TZANELA
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VARVARA VLASSOPOULOU
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NICHOLAOS THALASSINOS
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MICHAEL KOUTSILIERIS
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  • For correspondence: mkouts@medscape.com
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Abstract

Background: The prognostic significance of molecular staging using thyroglobulin (Tg)-based RT-PCR analysis in the peripheral blood of patients with differentiated thyroid cancer (DTC) was investigated. Herein, the specificity of molecular staging in DTC patients after thyroidectomy and 131I ablation therapy was analysed, using different specific cDNA primers in qualitative nested and classical RT-PCR protocols. Patients and Methods: Ninty-five patients receiving TSH suppressive therapy were included, of whom 51 were disease-free and 44 presented residual/recurrent or metastatic disease as evidenced by biochemical and/or 131I whole-body scan. Results: Our analysis detected RT-PCR products suggestive of the presence of Tg mRNA in all the blood samples tested, including healthy controls. A splice variant (350 bp) of Tg transcript (missing exon 3) was detected by nested RT-PCR in 34% of blood samples, however, without correlation to disease status. Conclusion: Qualitative Tg-based molecular staging in the peripheral blood of patients with DTC shows poor specificity in assessing DTC disease status.

  • Thyroglobulin
  • RT-PCR
  • thyroid cancer

Footnotes

  • Received January 25, 2005.
  • Revision received May 25, 2005.
  • Accepted May 26, 2005.
  • Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Anticancer Research: 25 (4)
Anticancer Research
Vol. 25, Issue 4
1 Jul 2005
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Molecular Staging Using Qualitative RT-PCR Analysis Detecting Thyreoglobulin mRNA in the Peripheral Blood of Patients with Differentiated Thyroid Cancer After Therapy
NIKI KARAVITAKI, PETER LEMBESSIS, MARINELA TZANELA, VARVARA VLASSOPOULOU, NICHOLAOS THALASSINOS, MICHAEL KOUTSILIERIS
Anticancer Research Jul 2005, 25 (4) 3135-3142;

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Molecular Staging Using Qualitative RT-PCR Analysis Detecting Thyreoglobulin mRNA in the Peripheral Blood of Patients with Differentiated Thyroid Cancer After Therapy
NIKI KARAVITAKI, PETER LEMBESSIS, MARINELA TZANELA, VARVARA VLASSOPOULOU, NICHOLAOS THALASSINOS, MICHAEL KOUTSILIERIS
Anticancer Research Jul 2005, 25 (4) 3135-3142;
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