Abstract
Background: Transitional cell carcinoma (TCC) in dogs is associated with high morbidity and mortality. Calcitriol and its analog seocalcitol, combined with medium-chain triglyceride (MCT), have potential for the treatment of this disease. Materials and Methods: TCC cells were treated with calcitriol or seocalcitol, alone or combined with MCT. Cell growth, cell cycle kinetics, vitamin D receptor (VDR) localization and expression, and Bcl-2 expression were measured. Results: Canine TCC expresses high levels of nuclear VDR. Furthermore, calcitriol and seocalcitol significantly inhibited cell growth and calcitriol caused G0/G1 cell cycle arrest. Bcl-2 expression was slightly decreased in cells treated with these compounds, although no significant changes in VDR expression were observed. MCT enhanced the growth inhibitory effect of both compounds. Conclusion: Calcitriol and seocalcitol inhibited TCC cell growth via induction of cell cycle arrest and MCT enhanced this effect. Therefore, calcitriol and seocalcitol with MCT may have therapeutic potential for canine bladder cancer.
- Vitamin D
- calcitriol
- vitamin D analog
- seocalcitol
- medium-chain triglyceride
- canine transitional cell carcinoma
- growth inhibition
- cell cycle
- VDR
Footnotes
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Abbreviations: Calcitriol, 1α, 25-dihydroxyvitamin D3; Seocalcitol, 22, 24-diene-24a,26a,27a-trihomo-1α, 25-dihydroxyvitamin D3; MCT, medium-chain triglyceride; TCC cell line, canine transitional cell carcinoma cell line.
- Received May 9, 2005.
- Accepted May 18, 2005.
- Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved