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Research ArticleExperimental Studies

UGT1A10 is Responsible for SN-38 Glucuronidation and its Expression in Human Lung Cancers

TETSUYA OGURI, TOSHIAKI TAKAHASHI, MITSURU MIYAZAKI, TAKESHI ISOBE, NOBUOKI KOHNO, PETER I. MACKENZIE and YASUHIRO FUJIWARA
Anticancer Research September 2004, 24 (5A) 2893-2896;
TETSUYA OGURI
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TOSHIAKI TAKAHASHI
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MITSURU MIYAZAKI
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TAKESHI ISOBE
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NOBUOKI KOHNO
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PETER I. MACKENZIE
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YASUHIRO FUJIWARA
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Abstract

Background: We previously reported that up-regulation of glucuronidation activity catalyzed by uridine 5’diphosphoglucuronosyltransferase (UGT) is one of the mechanisms associated with irinotecan hydrochloride/7-ethyl-10-hydroxycamptothecin (CPT-11/SN-38) resistance. In order to extend this result to the clinical setting, it is important to elucidate the role of SN-38 glucuronidation by UGT1A isoforms in CPT-11/SN-38 resistance in vivo. Materials and Methods: We examined SN-38 glucuronidation activity in COS-7 cells transfected with full-length cDNAs for human UGT isoforms (UGT1A1, UGT1A3, UGT1A6 and UGT1A10). The gene expression levels of UGT isoforms were examined in lung cancer cell lines and 14 lung cancer samples by semiquantitative RT-PCR. Results: Our HPLC assay results showed that both UGT1A1 and UGT1A10 are responsible for SN-38 glucuronidation. The levels of UGT1A1 and UGT1A10 expression in a CPT-11/SN-38-resistant cell line were increased compared to levels in the parent cell line. Furthermore, there was considerable intersubject variability in 14 lung cancer samples, but UGT1A1 and UGT1A10 expression levels were significantly correlated (r=0.70, p=0.004). Our results suggest that not only UGT 1A1, but also UGT 1A10, plays an important role in detoxifying CPT-11/SN-38, leading to resistance to CPT-11/SN-38 in lung cancer.

Footnotes

    • Received February 24, 2004.
    • Accepted June 30, 2004.
  • Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Anticancer Research: 24 (5A)
Anticancer Research
Vol. 24, Issue 5A
September-October 2004
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UGT1A10 is Responsible for SN-38 Glucuronidation and its Expression in Human Lung Cancers
TETSUYA OGURI, TOSHIAKI TAKAHASHI, MITSURU MIYAZAKI, TAKESHI ISOBE, NOBUOKI KOHNO, PETER I. MACKENZIE, YASUHIRO FUJIWARA
Anticancer Research Sep 2004, 24 (5A) 2893-2896;

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UGT1A10 is Responsible for SN-38 Glucuronidation and its Expression in Human Lung Cancers
TETSUYA OGURI, TOSHIAKI TAKAHASHI, MITSURU MIYAZAKI, TAKESHI ISOBE, NOBUOKI KOHNO, PETER I. MACKENZIE, YASUHIRO FUJIWARA
Anticancer Research Sep 2004, 24 (5A) 2893-2896;
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