Absence of BRAF Gene Mutations Differentiates Spitz Nevi from Malignant Melanoma

Abstract

Background: Distinction of Spitz nevus from malignant melanoma is sometimes difficult on the basis of conventional histology. A high rate of BRAF gene mutations in malignant melanomas (66%) and nevi (82%) has recently been reported. Materials and Methods: We screened a series of 20 Spitz nevi for BRAF mutations in exons 11 and 15 by denaturing gradient gel electrophoresis (DGGE). Results: BRAF mutations could not be identified in Spitz nevi. Conclusion: Our results show that mutations within the BRAF gene are useful markers for the differential diagnosis between Spitz nevus and malignant melanoma.