Abstract
Objectives: Nuclear factor-kB (NF-kB) is a transcription factor that participates in the induction of several genes for cytokines and enzymes that play important functional roles in various cell types. The aim of this study was to determine NF-kB activation status in human colorectal carcinoma and its correlation to the clinicopathological characteristics of patients. Materials and Methods: We examined the activation status of NF-kB in 28 resected colorectal carcinomas and in colonic mucosa from uninvolved portions of these specimens by electrophoretic mobility shift assay (EMSA) and immunohistochemical staining for the p65 subunit of NF-kB. Results: EMSA showed much greater activation in the tumors than in normal mucosa, as did epithelial p65 immunostaining. NF-kB activation significantly increased in the more progressed cases (T3+T4 cases or Stage II< cases). In vitro studies using lipopolysaccharide (LPS)-responsive colon carcinoma cells suggested a correlation between NF-kB activation and cell proliferation. Conclusion: Our findings indicated that NF-kB is constitutively activated in human colorectal carcinoma tissue and correlates with tumor progression. The regulation of this transcription factor might be therapeutically useful against these tumors.
Footnotes
- Received November 14, 2003.
- Accepted February 4, 2004.
- Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
Jump to section
Related Articles
Cited By...
- Incremental Elevations in TNF{alpha} and IL6 in the Human Colon and Procancerous Changes in the Mucosal Transcriptome Accompany Adiposity
- Nucleoside Diphosphate Kinase-3 (NME3) Enhances TLR5-Induced NF{kappa}B Activation
- Downregulation of SAFB Sustains the NF-{kappa}B Pathway by Targeting TAK1 during the Progression of Colorectal Cancer
- Vitamin D, Inflammation, and Colorectal Cancer Progression: A Review of Mechanistic Studies and Future Directions for Epidemiological Studies
- The Combination of Nuclear Factor Kappa B, Cyclo-oxygenase-2 and Vascular Endothelial Growth Factor Expression Predicts Poor Prognosis in Stage II and III Colorectal Cancer
- Nimbolide, a Limonoid Triterpene, Inhibits Growth of Human Colorectal Cancer Xenografts by Suppressing the Proinflammatory Microenvironment
- Curcumin Sensitizes Human Colorectal Cancer Xenografts in Nude Mice to {gamma}-Radiation by Targeting Nuclear Factor-{kappa}B-Regulated Gene Products
- A Novel Variant of Ileal Bile Acid Binding Protein Is Up-regulated through Nuclear Factor-{kappa}B Activation in Colorectal Adenocarcinoma
- Nuclear Factor-kB Tumor Expression Predicts Response and Survival in Irinotecan-Refractory Metastatic Colorectal Cancer Treated With Cetuximab-Irinotecan Therapy
- Nuclear Factor-{kappa}B in Development, Prevention, and Therapy of Cancer
- The Putative Tumor Suppressor Cdx2 Is Overexpressed by Human Colorectal Adenocarcinomas