Skip to main content

Main menu

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Subscribers
    • Advertisers
    • Editorial Board
    • Special Issues 2025
  • Journal Metrics
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Anticancer Research
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Anticancer Research

Advanced Search

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Subscribers
    • Advertisers
    • Editorial Board
    • Special Issues 2025
  • Journal Metrics
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Visit us on Facebook
  • Follow us on Linkedin
Research ArticleExperimental Studies

In-silico Analysis of Kallikrein Gene Expression in Pancreatic and Colon Cancers

GEORGE M. YOUSEF, CARLA A. BORGONO, CYNTHIA POPALIS, GEORGE M. YACOUB, MARY-ELLEN POLYMERIS, ANTONINUS SOOSAIPILLAI and ELEFTHERIOS P. DIAMANDIS
Anticancer Research January 2004, 24 (1) 43-52;
GEORGE M. YOUSEF
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
CARLA A. BORGONO
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
CYNTHIA POPALIS
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
GEORGE M. YACOUB
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
MARY-ELLEN POLYMERIS
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
ANTONINUS SOOSAIPILLAI
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
ELEFTHERIOS P. DIAMANDIS
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Human kallikreins are a cluster of 15 serine protease genes located in the chromosomal band 19q13.4, a non-randomly rearranged region in many solid tumors, including pancreatic cancer. We utilized the SAGE and EST databases of the Cancer Genome Anatomy Project to perform in-silico analysis of kallikrein gene expression in normal and cancerous pancreatic and colon tissues and cell lines using virtual Northern blotting (VNB), digital differential display (DDD) and X-profiler. At least two kallikreins, KLK6 and KLK10, are significantly up-regulated in pancreatic cancer. We probed 2 normal and 6 pancreatic cancer SAGE libraries with gene-specific tags for each of these kallikreins. KLK6 was found to be expressed in 5/6 cancer libraries and showed the most marked (5-fold) increase in average expression levels in cancer vs. normal. These data were verified by screening the EST databases, where all mRNA clones isolated were from cancerous libraries, with no clones detected in normal pancreatic tissues or cell lines. X-profiler comparison of two pools of normal and cancerous pancreatic libraries further verified the significant increase of KLK6 expression levels in pancreatic cancer. DDD data showed a 13-fold increase in KLK10 expression in pancreatic cancer. Three kallikrein genes, KLK6, 8 and 10 are overexpressed in colon cancer compared to normal colon, while one kallikrein, KLK1, is downregulated. While no expression of KLK6 was detected in normal colon, KLK6-specific tags were detectable in 2 cancer libraries. Similar results were obtained by EST screening; no KLK6 clones were detected in any of the 28 normal libraries examined, while 10 KLK6 EST clones were found in colon adenocarcinoma. KLK10 was not detectable in normal colon. Gene-specific tags were, however, detectable with high density in colon cancer and 7 EST clones were found to be expressed in colon Adenocarcinoma.

  • Received October 20, 2003.
  • Accepted December 18, 2003.
  • Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
PreviousNext
Back to top

In this issue

Anticancer Research: 24 (1)
Anticancer Research
Vol. 24, Issue 1
January-February 2004
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Ed Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Anticancer Research.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
In-silico Analysis of Kallikrein Gene Expression in Pancreatic and Colon Cancers
(Your Name) has sent you a message from Anticancer Research
(Your Name) thought you would like to see the Anticancer Research web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
13 + 2 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
In-silico Analysis of Kallikrein Gene Expression in Pancreatic and Colon Cancers
GEORGE M. YOUSEF, CARLA A. BORGONO, CYNTHIA POPALIS, GEORGE M. YACOUB, MARY-ELLEN POLYMERIS, ANTONINUS SOOSAIPILLAI, ELEFTHERIOS P. DIAMANDIS
Anticancer Research Jan 2004, 24 (1) 43-52;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
In-silico Analysis of Kallikrein Gene Expression in Pancreatic and Colon Cancers
GEORGE M. YOUSEF, CARLA A. BORGONO, CYNTHIA POPALIS, GEORGE M. YACOUB, MARY-ELLEN POLYMERIS, ANTONINUS SOOSAIPILLAI, ELEFTHERIOS P. DIAMANDIS
Anticancer Research Jan 2004, 24 (1) 43-52;
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • Targeted and Explorative Profiling of Kallikrein Proteases and Global Proteome Biology of Pancreatic Ductal Adenocarcinoma, Chronic Pancreatitis, and Normal Pancreas Highlights Disease-Specific Proteome Remodelling
  • The Effect of Productive HPV16 Infection on Global Gene Expression of Cervical Epithelium
  • Effect of Productive Human Papillomavirus 16 Infection on Global Gene Expression in Cervical Epithelium
  • Integrated Genomic Characterization of the Kallikrein Gene Locus in Cancer
  • Digital Transcript Profile Analysis with aRNA-LongSAGE Validates FERMT1 As a Potential Novel Prognostic Marker for Colon Cancer
  • Utility of Kallikrein-Related Peptidases (KLKs) as Cancer Biomarkers
  • Kallikrein 6 Induces E-Cadherin Shedding and Promotes Cell Proliferation, Migration, and Invasion
  • Proteinase-activated Receptors, Targets for Kallikrein Signaling
  • Clinicopathologic and Biological Significance of Kallikrein 6 Overexpression in Human Gastric Cancer
  • Clinical Significance of Human Kallikrein Gene 6 Messenger RNA Expression in Colorectal Cancer
  • Google Scholar

More in this TOC Section

  • Tissue Prognostic Markers for Clear Cell Renal Cell Carcinoma Tumor-stroma Interaction: Impact on TNM Staging Parameters
  • CA9-Positive Keloid Fibroblasts: Modulator of Local Inflammation Induced by the Hypoxic and Glycolysis-enhanced Microenvironment
  • Predictive Factors for Multiple Metastases of Clear-cell Renal Cell Carcinoma
Show more Experimental Studies

Similar Articles

Anticancer Research

© 2025 Anticancer Research

Powered by HighWire