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Research ArticleExperimental Studies

Drug-induced Caspase-3 Activation in a Ewing Tumor Cell Line and Primary Ewing Tumor Cells

CHRISTINE MAUZ-KÖRHOLZ, MARTIN KACHEL, BRITT HARMS-SCHIRRA, ANNE KLEIN-VEHNE, PETER-ULRICH TUNN and DIETER KÖRHOLZ
Anticancer Research January 2004, 24 (1) 145-150;
CHRISTINE MAUZ-KÖRHOLZ
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MARTIN KACHEL
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BRITT HARMS-SCHIRRA
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ANNE KLEIN-VEHNE
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PETER-ULRICH TUNN
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DIETER KÖRHOLZ
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Abstract

Ewing sarcoma is a rare malignancy occurring in childhood and adolescence which has a prognosis of about 50% long-term event-free survival, depending on risk factors such as tumor volume, initial metastases at the time of diagnosis and tumor response to presurgical chemotherapy. In order to tailor therapy to the individual patient, new prognostic factors need to be identified. In this study, we showed that etoposide and actinomycin D kill Ewing tumor cells (RD-ES cell line) mainly via a caspase-dependent mechanism. Both drugs induced a significant caspase-3 activation, which can be detected with a new caspase-3 assay. Dose-response analyses showed that induction of cell death and caspase-3 activation is mediated by similar concentration ranges of both drugs. In addition to the cell line, caspase-3 activation was also shown during drug-mediated stimulation of freshly isolated cells from tumor biopsies. In conclusion, actinomycin D and etoposide stimulated caspase-3 activation in a Ewing tumor cell line and in freshly isolated tumor cells, causing drug-induced cell death. Thus, determination of caspase-3 activation might be a suitable method for drug sensitivity testing in patients with Ewing tumors before initiating treatment. This assay could therefore help individualize therapy in future tumor patients.

  • Received August 19, 2003.
  • Accepted October 13, 2003.
  • Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Anticancer Research: 24 (1)
Anticancer Research
Vol. 24, Issue 1
January-February 2004
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Drug-induced Caspase-3 Activation in a Ewing Tumor Cell Line and Primary Ewing Tumor Cells
CHRISTINE MAUZ-KÖRHOLZ, MARTIN KACHEL, BRITT HARMS-SCHIRRA, ANNE KLEIN-VEHNE, PETER-ULRICH TUNN, DIETER KÖRHOLZ
Anticancer Research Jan 2004, 24 (1) 145-150;

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Drug-induced Caspase-3 Activation in a Ewing Tumor Cell Line and Primary Ewing Tumor Cells
CHRISTINE MAUZ-KÖRHOLZ, MARTIN KACHEL, BRITT HARMS-SCHIRRA, ANNE KLEIN-VEHNE, PETER-ULRICH TUNN, DIETER KÖRHOLZ
Anticancer Research Jan 2004, 24 (1) 145-150;
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