Frequent CDH3 Demethylation in Advanced Gastric Carcinoma
- KENJI HIBI,
- YO-HEI KITAMURA,
- HIROKI MIZUKAMI,
- TETSUHIRO GOTO,
- KAZUMA SAKURABA,
- MAKIKO SAKATA,
- MITSUO SAITO,
- KAZUYOSHI ISHIBASHI,
- GAKU KIGAWA,
- HIROSHI NEMOTO and
- YUTAKA SANADA
- Correspondence to: Kenji Hibi, Department of Surgery, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Aoba-ku, Yokohama 227-8501, Japan. Tel: +81 459711151, Fax: +81 459717125, e-mail: kenjih-ngy{at}umin.ac.jp
Abstract
Background: We recently found that CDH3 was frequently demethylated in advanced colorectal carcinomas. This prompted us to examine the demethylation status of the CDH3 gene in gastric carcinomas. Materials and Methods: The demethylation status of the CDH3 gene was examined in primary tumors derived from 36 patients with gastric carcinoma using a quantitative methylation-specific PCR (qMSP) and was evaluated the correlation between the demethylation status and the clinicopathological findings. Results. Demethylation of the CDH3 gene was detected in 25 out of the 36 (69%) primary gastric carcinomas, suggesting that the aberrant demethylation of CDH3 is a frequent event in gastric carcinomas. Demethylation of CDH3 was significantly associated with increasing TNM stage (p=0.0261). Moreover, a trend was shown toward infiltration beyond the serosa being associated with demethylationof CDH3 (p=0.0733). Conclusion: CDH3 was frequently demethylated in advanced gastric carcinomas.
- Received February 9, 2009.
- Revision received June 30, 2009.
- Accepted July 14, 2009.
- Copyright© 2009 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
