Abstract
Background/Aim: Gallbladder cancers are well known to frequently exhibit TP53 as well as K-ras gene mutations. This study performed a TP53 gene investigation by PCR-SSCP and direct sequencing using both bile supernatants and tissue samples from cholecystectomy specimens lacking gallbladder cancer, in order to investigate gallbladder carcinogenesis. Materials and Methods: Eighteen out of 294 cases, mainly of cholecystitis, were extracted by screening SSCP of bile supernatants for TP53 mutations, and investigation of their tissue samples both SSCP and direct sequencing. Results: Non-neoplastic mucosal samples demonstrated shifted bands in 11 cases (61%), and mutations were confirmed in 7 cases (64%). Unexpectedly, no cases showed identical point mutations in both bile and tissue samples. G:C to A:T transitions, thought to be sporadic mutations, predominated (77%). Conclusion: Sporadic TP53 transition mutations were demonstrated in non-neoplastic lesions such as severe cholecystitis, indicating an importance for a chronic cholecystitis-carcinoma sequence in gallbladder carcinogenesis.
- Received August 24, 2010.
- Revision received October 14, 2010.
- Accepted October 15, 2010.
- Copyright© 2010 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved